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1994-06-25
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44 lines
Document 0668
DOCN M9460668
TI B cell IL-7. Human B cell lines constitutively secrete IL-7 and express
IL-7 receptors.
DT 9408
AU Benjamin D; Sharma V; Knobloch TJ; Armitage RJ; Dayton MA; Goodwin RG;
Division of Hematology and Oncology, Arthur G. James Cancer; Hospital,
Columbus, OH.
SO J Immunol. 1994 May 15;152(10):4749-57. Unique Identifier : AIDSLINE
MED/94230951
AB IL-7 was originally reported as a cytokine produced by stromal cells
which supports pre-B cell proliferation in vitro. To determine whether
human B cells secrete IL-7 and express IL-7R we studied a wide panel of
B cell lines (CLS). In Northern blot analysis we detected 2.4 kb IL-7
mRNA and by quantitative PCR we demonstrated IL-7 expression in 5 of 6 B
CLS derived from patients with AIDS-associated Burkitt's lymphoma
(AABCL), 3 of 3 CLS derived from patients with American Burkitt's
lymphoma and 5 of 6 normal lymphoblastoid CLS. Only 1 of 5 African
Burkitt's lymphoma CLS and 2 of 7 EBV- CLS expressed IL-7. A total of
484-bp amplicons was cloned and sequenced and found to correspond to the
original IL-7 sequence. Constitutive IL-7 secretion was detected in 5 of
6 AABCL and in 6 of 7 normal lymphoblastoid CLS but in none of the 7
EBV- CLS. IL-7R expression was demonstrated in 8 of 26 CLS, none of
which secreted IL-7. Our data suggest that 1) IL-7 mRNA is expressed in
malignant B cell phenotypes, which correspond to a narrow window in the
B cell differentiation pathway (pre-B, early B, intermediate B), as well
as in normal lymphoblastoid B CLS. 2) IL-7 mRNA is expressed in both
EBV+ and EBV- CLS, but only the EBV+ CLS secrete IL-7. 3) B cells
activated by both EBV and HIV-1 (AABCL) secrete the greatest amount of
IL-7. 4) IL-7 autocrine loops are not evident since IL-7R were detected
on on CLS, which do not secrete IL-7. Our data provide the first direct
evidence of IL-7 secretion by human cells and it is yet to be determined
whether IL-7 is secreted by other cell types.
DE B-Lymphocytes/CHEMISTRY/*METABOLISM/SECRETION Base Sequence Blotting,
Northern Cell Line Human
Interleukin-7/BIOSYNTHESIS/GENETICS/*SECRETION Molecular Sequence Data
Polymerase Chain Reaction Receptors, Interleukin/*ANALYSIS RNA,
Messenger/ANALYSIS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).